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Heidolph Instruments heidolph titramax 1000
Heidolph Titramax 1000, supplied by Heidolph Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/titramax+1000/1000+titramax/bio_rxiv__64898__2026__04__30__722052-388-26-26
Average 86 stars, based on 1 article reviews
heidolph titramax 1000 - by Bioz Stars, 2026-10
86/100 stars

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Incubation:

Article Title: Modulators of gene amplification alter evolution of antibiotic resistance in Staphylococcus aureus
Article Snippet: For experiments in liquid media, bacteria were grown at 37 o C, shaking at 300 rpm in modified M63 media (13.6 g/L KH 2 PO 4 , 2g/L (NH 4 ) 2 SO 4 , 0.4 μM ferric citrate, 1mM MgSO 4 ; pH adjusted to 7.0 with KOH) supplemented with 0.3% glucose, 0.1 μg/mL biotin, 2 μg/mL nicotinic acid, 1 × Supplement EZ (Teknova) and 1 × ACGU solution (Teknova) [ ]. .. For experiments with 96-well or deep well plates, the plates were incubated in a Titramax 1000 (Heidolph) incubator at 37 o C shaking at 900 rpm. .. To construct and mutate plasmids and strains, cells were grown in LB liquid medium (10 g/L bacto-tryptone, 5 g/L yeast extract, 10 g/L NaCl) at 25 o C, 30 o C, or 37 o C as indicated, shaking at 300 rpm, or on LB plates (15 g/L agar) supplemented with the appropriate antibiotics (10 μg/mL chloramphenicol, 50 μg/mL kanamycin, 10 μg/mL trimethoprim, 10 μg/mL erythromycin) or 0.4% para-chlorophenylalanine (PCPA).

Article Title: GRN_modeler: An Intuitive Tool for Constructing and Evaluating Gene Regulatory Networks and its Applications to Oscillators and a Light Biosensor
Article Snippet: For each condition 200 μ L were added to a 96-well plate (Greiner, REF #655090). .. The 96-well plate was placed on top of a LITOS light device [ ] and incubated at room temperature under agitation at 450 rpm (Titramax 1000, heidolph). ..

Article Title: Ex vivo-stimulated cytokine expression in whole blood indicates swine influenza vaccine-induced immune memory.
Article Snippet: Swine influenza viruses (SwIAV) pose a zoonotic threat and contribute to interspecies viral reassortment.. Effective vaccination strategies are essential, yet accessible correlates of protection remain limited.. We simplified a whole blood (WB) assay to assess immune memory ex vivo in pigs vaccinated with Respiporc FLU3® (n = 6) versus placebo controls (n = 6), followed by homologous challenge with SwIAV (H1avN1).

Article Title: Development, in vitro and ex vivo characterization of lamotrigine-loaded bovine serum albumin nanoparticles using QbD approach.
Article Snippet: In vitro permeability measurements to evaluate the permeability (Pe, cm/s) of the initial suspension and the selected formulations, the parallel artificial membrane permeability assay specific to the blood-brain barrier (BBB-PaMPa) was employed (Katona et al., 2020). a 10 mM laM solution was prepared in dimethylsulfoxide (DMsO), which was further diluted with sNes to obtain the 100 μM reference donor solution. the filter donor plate (MultiscreentM-iP, MaiPN4510, pore size 0.45 μm; Millipore, Merck ltd., Budapest, hungary) was coated with 5 μl of lipid solution (12 mg of porcine brain polar lipid extract dissolved in 420 μl hexane and 180 μl dodecane). the donor plate was inserted to the acceptor plate (Multiscreen acceptor Plate, MssaccePtOR; Millipore, Merck ltd., Budapest, hungary), which contained 300 μl of DPBs solution (ph 7.4). .. 150-150 μl of reference laM solution, as well as of the selected formulations redispersed with sNes (with a nominal concentration of 2 mg/ml of laM) were transferred to the donor plate lipid membrane. the sandwich system was incubated at 37 °c for 4 h (heidolph titramax 1000, heidolph instruments, schwabach, Germany). then, the PaMPa sandwich plates were separated and the laM concentrations in the donor and acceptor chambers were determined using RP-hPlc-DaD. the effective permeability and membrane retention of the drugs were calculated using the following eq. (3): P V A t C t Se A ss A 2 303 1 . . . log (3) where Pe is the effective permeability coefficient (cm/s), A is the filter area (0.24 cm2), VA is the volume of the acceptor phase (0.3 cm3), t is the incubation time (s), τSS is the time to reach steady state (s), CA(t) is the concentration of the compound in the acceptor phase at time point t (mol/cm3), and S is laM solubility in the donor phase. the solubility of laM in donor phase was determined after centrifugation (at 14,000 rpm, 15 minutes, eppendorf centrifuge 5804 R, thermo scientificTM, Waltham, Ma, Usa) in Microcon centrifugal Filter Devices (30,000 molecular weight (MWcO)) and 5× dilution of formulations in MeOh, using the same RP-hPlc-DaD system. the flux of the samples was calculated using the following eq. (4): Flux P S e = . (4) For each assay, six parallel measurements were made and data were presented as means ± sD. ..

other:

Article Title:
Article Snippet: The resulting plate was incubated overnight at 37 °C shaking at 900 rpm (Titramax 1000 & Incubator 1000, Heidolph).

Concentration Assay:

Article Title: Development, in vitro and ex vivo characterization of lamotrigine-loaded bovine serum albumin nanoparticles using QbD approach.
Article Snippet: In vitro permeability measurements to evaluate the permeability (Pe, cm/s) of the initial suspension and the selected formulations, the parallel artificial membrane permeability assay specific to the blood-brain barrier (BBB-PaMPa) was employed (Katona et al., 2020). a 10 mM laM solution was prepared in dimethylsulfoxide (DMsO), which was further diluted with sNes to obtain the 100 μM reference donor solution. the filter donor plate (MultiscreentM-iP, MaiPN4510, pore size 0.45 μm; Millipore, Merck ltd., Budapest, hungary) was coated with 5 μl of lipid solution (12 mg of porcine brain polar lipid extract dissolved in 420 μl hexane and 180 μl dodecane). the donor plate was inserted to the acceptor plate (Multiscreen acceptor Plate, MssaccePtOR; Millipore, Merck ltd., Budapest, hungary), which contained 300 μl of DPBs solution (ph 7.4). .. 150-150 μl of reference laM solution, as well as of the selected formulations redispersed with sNes (with a nominal concentration of 2 mg/ml of laM) were transferred to the donor plate lipid membrane. the sandwich system was incubated at 37 °c for 4 h (heidolph titramax 1000, heidolph instruments, schwabach, Germany). then, the PaMPa sandwich plates were separated and the laM concentrations in the donor and acceptor chambers were determined using RP-hPlc-DaD. the effective permeability and membrane retention of the drugs were calculated using the following eq. (3): P V A t C t Se A ss A 2 303 1 . . . log (3) where Pe is the effective permeability coefficient (cm/s), A is the filter area (0.24 cm2), VA is the volume of the acceptor phase (0.3 cm3), t is the incubation time (s), τSS is the time to reach steady state (s), CA(t) is the concentration of the compound in the acceptor phase at time point t (mol/cm3), and S is laM solubility in the donor phase. the solubility of laM in donor phase was determined after centrifugation (at 14,000 rpm, 15 minutes, eppendorf centrifuge 5804 R, thermo scientificTM, Waltham, Ma, Usa) in Microcon centrifugal Filter Devices (30,000 molecular weight (MWcO)) and 5× dilution of formulations in MeOh, using the same RP-hPlc-DaD system. the flux of the samples was calculated using the following eq. (4): Flux P S e = . (4) For each assay, six parallel measurements were made and data were presented as means ± sD. ..

Membrane:

Article Title: Development, in vitro and ex vivo characterization of lamotrigine-loaded bovine serum albumin nanoparticles using QbD approach.
Article Snippet: In vitro permeability measurements to evaluate the permeability (Pe, cm/s) of the initial suspension and the selected formulations, the parallel artificial membrane permeability assay specific to the blood-brain barrier (BBB-PaMPa) was employed (Katona et al., 2020). a 10 mM laM solution was prepared in dimethylsulfoxide (DMsO), which was further diluted with sNes to obtain the 100 μM reference donor solution. the filter donor plate (MultiscreentM-iP, MaiPN4510, pore size 0.45 μm; Millipore, Merck ltd., Budapest, hungary) was coated with 5 μl of lipid solution (12 mg of porcine brain polar lipid extract dissolved in 420 μl hexane and 180 μl dodecane). the donor plate was inserted to the acceptor plate (Multiscreen acceptor Plate, MssaccePtOR; Millipore, Merck ltd., Budapest, hungary), which contained 300 μl of DPBs solution (ph 7.4). .. 150-150 μl of reference laM solution, as well as of the selected formulations redispersed with sNes (with a nominal concentration of 2 mg/ml of laM) were transferred to the donor plate lipid membrane. the sandwich system was incubated at 37 °c for 4 h (heidolph titramax 1000, heidolph instruments, schwabach, Germany). then, the PaMPa sandwich plates were separated and the laM concentrations in the donor and acceptor chambers were determined using RP-hPlc-DaD. the effective permeability and membrane retention of the drugs were calculated using the following eq. (3): P V A t C t Se A ss A 2 303 1 . . . log (3) where Pe is the effective permeability coefficient (cm/s), A is the filter area (0.24 cm2), VA is the volume of the acceptor phase (0.3 cm3), t is the incubation time (s), τSS is the time to reach steady state (s), CA(t) is the concentration of the compound in the acceptor phase at time point t (mol/cm3), and S is laM solubility in the donor phase. the solubility of laM in donor phase was determined after centrifugation (at 14,000 rpm, 15 minutes, eppendorf centrifuge 5804 R, thermo scientificTM, Waltham, Ma, Usa) in Microcon centrifugal Filter Devices (30,000 molecular weight (MWcO)) and 5× dilution of formulations in MeOh, using the same RP-hPlc-DaD system. the flux of the samples was calculated using the following eq. (4): Flux P S e = . (4) For each assay, six parallel measurements were made and data were presented as means ± sD. ..

PAMPA Assay:

Article Title: Development, in vitro and ex vivo characterization of lamotrigine-loaded bovine serum albumin nanoparticles using QbD approach.
Article Snippet: In vitro permeability measurements to evaluate the permeability (Pe, cm/s) of the initial suspension and the selected formulations, the parallel artificial membrane permeability assay specific to the blood-brain barrier (BBB-PaMPa) was employed (Katona et al., 2020). a 10 mM laM solution was prepared in dimethylsulfoxide (DMsO), which was further diluted with sNes to obtain the 100 μM reference donor solution. the filter donor plate (MultiscreentM-iP, MaiPN4510, pore size 0.45 μm; Millipore, Merck ltd., Budapest, hungary) was coated with 5 μl of lipid solution (12 mg of porcine brain polar lipid extract dissolved in 420 μl hexane and 180 μl dodecane). the donor plate was inserted to the acceptor plate (Multiscreen acceptor Plate, MssaccePtOR; Millipore, Merck ltd., Budapest, hungary), which contained 300 μl of DPBs solution (ph 7.4). .. 150-150 μl of reference laM solution, as well as of the selected formulations redispersed with sNes (with a nominal concentration of 2 mg/ml of laM) were transferred to the donor plate lipid membrane. the sandwich system was incubated at 37 °c for 4 h (heidolph titramax 1000, heidolph instruments, schwabach, Germany). then, the PaMPa sandwich plates were separated and the laM concentrations in the donor and acceptor chambers were determined using RP-hPlc-DaD. the effective permeability and membrane retention of the drugs were calculated using the following eq. (3): P V A t C t Se A ss A 2 303 1 . . . log (3) where Pe is the effective permeability coefficient (cm/s), A is the filter area (0.24 cm2), VA is the volume of the acceptor phase (0.3 cm3), t is the incubation time (s), τSS is the time to reach steady state (s), CA(t) is the concentration of the compound in the acceptor phase at time point t (mol/cm3), and S is laM solubility in the donor phase. the solubility of laM in donor phase was determined after centrifugation (at 14,000 rpm, 15 minutes, eppendorf centrifuge 5804 R, thermo scientificTM, Waltham, Ma, Usa) in Microcon centrifugal Filter Devices (30,000 molecular weight (MWcO)) and 5× dilution of formulations in MeOh, using the same RP-hPlc-DaD system. the flux of the samples was calculated using the following eq. (4): Flux P S e = . (4) For each assay, six parallel measurements were made and data were presented as means ± sD. ..

Permeability:

Article Title: Development, in vitro and ex vivo characterization of lamotrigine-loaded bovine serum albumin nanoparticles using QbD approach.
Article Snippet: In vitro permeability measurements to evaluate the permeability (Pe, cm/s) of the initial suspension and the selected formulations, the parallel artificial membrane permeability assay specific to the blood-brain barrier (BBB-PaMPa) was employed (Katona et al., 2020). a 10 mM laM solution was prepared in dimethylsulfoxide (DMsO), which was further diluted with sNes to obtain the 100 μM reference donor solution. the filter donor plate (MultiscreentM-iP, MaiPN4510, pore size 0.45 μm; Millipore, Merck ltd., Budapest, hungary) was coated with 5 μl of lipid solution (12 mg of porcine brain polar lipid extract dissolved in 420 μl hexane and 180 μl dodecane). the donor plate was inserted to the acceptor plate (Multiscreen acceptor Plate, MssaccePtOR; Millipore, Merck ltd., Budapest, hungary), which contained 300 μl of DPBs solution (ph 7.4). .. 150-150 μl of reference laM solution, as well as of the selected formulations redispersed with sNes (with a nominal concentration of 2 mg/ml of laM) were transferred to the donor plate lipid membrane. the sandwich system was incubated at 37 °c for 4 h (heidolph titramax 1000, heidolph instruments, schwabach, Germany). then, the PaMPa sandwich plates were separated and the laM concentrations in the donor and acceptor chambers were determined using RP-hPlc-DaD. the effective permeability and membrane retention of the drugs were calculated using the following eq. (3): P V A t C t Se A ss A 2 303 1 . . . log (3) where Pe is the effective permeability coefficient (cm/s), A is the filter area (0.24 cm2), VA is the volume of the acceptor phase (0.3 cm3), t is the incubation time (s), τSS is the time to reach steady state (s), CA(t) is the concentration of the compound in the acceptor phase at time point t (mol/cm3), and S is laM solubility in the donor phase. the solubility of laM in donor phase was determined after centrifugation (at 14,000 rpm, 15 minutes, eppendorf centrifuge 5804 R, thermo scientificTM, Waltham, Ma, Usa) in Microcon centrifugal Filter Devices (30,000 molecular weight (MWcO)) and 5× dilution of formulations in MeOh, using the same RP-hPlc-DaD system. the flux of the samples was calculated using the following eq. (4): Flux P S e = . (4) For each assay, six parallel measurements were made and data were presented as means ± sD. ..

Solubility:

Article Title: Development, in vitro and ex vivo characterization of lamotrigine-loaded bovine serum albumin nanoparticles using QbD approach.
Article Snippet: In vitro permeability measurements to evaluate the permeability (Pe, cm/s) of the initial suspension and the selected formulations, the parallel artificial membrane permeability assay specific to the blood-brain barrier (BBB-PaMPa) was employed (Katona et al., 2020). a 10 mM laM solution was prepared in dimethylsulfoxide (DMsO), which was further diluted with sNes to obtain the 100 μM reference donor solution. the filter donor plate (MultiscreentM-iP, MaiPN4510, pore size 0.45 μm; Millipore, Merck ltd., Budapest, hungary) was coated with 5 μl of lipid solution (12 mg of porcine brain polar lipid extract dissolved in 420 μl hexane and 180 μl dodecane). the donor plate was inserted to the acceptor plate (Multiscreen acceptor Plate, MssaccePtOR; Millipore, Merck ltd., Budapest, hungary), which contained 300 μl of DPBs solution (ph 7.4). .. 150-150 μl of reference laM solution, as well as of the selected formulations redispersed with sNes (with a nominal concentration of 2 mg/ml of laM) were transferred to the donor plate lipid membrane. the sandwich system was incubated at 37 °c for 4 h (heidolph titramax 1000, heidolph instruments, schwabach, Germany). then, the PaMPa sandwich plates were separated and the laM concentrations in the donor and acceptor chambers were determined using RP-hPlc-DaD. the effective permeability and membrane retention of the drugs were calculated using the following eq. (3): P V A t C t Se A ss A 2 303 1 . . . log (3) where Pe is the effective permeability coefficient (cm/s), A is the filter area (0.24 cm2), VA is the volume of the acceptor phase (0.3 cm3), t is the incubation time (s), τSS is the time to reach steady state (s), CA(t) is the concentration of the compound in the acceptor phase at time point t (mol/cm3), and S is laM solubility in the donor phase. the solubility of laM in donor phase was determined after centrifugation (at 14,000 rpm, 15 minutes, eppendorf centrifuge 5804 R, thermo scientificTM, Waltham, Ma, Usa) in Microcon centrifugal Filter Devices (30,000 molecular weight (MWcO)) and 5× dilution of formulations in MeOh, using the same RP-hPlc-DaD system. the flux of the samples was calculated using the following eq. (4): Flux P S e = . (4) For each assay, six parallel measurements were made and data were presented as means ± sD. ..

Centrifugation:

Article Title: Development, in vitro and ex vivo characterization of lamotrigine-loaded bovine serum albumin nanoparticles using QbD approach.
Article Snippet: In vitro permeability measurements to evaluate the permeability (Pe, cm/s) of the initial suspension and the selected formulations, the parallel artificial membrane permeability assay specific to the blood-brain barrier (BBB-PaMPa) was employed (Katona et al., 2020). a 10 mM laM solution was prepared in dimethylsulfoxide (DMsO), which was further diluted with sNes to obtain the 100 μM reference donor solution. the filter donor plate (MultiscreentM-iP, MaiPN4510, pore size 0.45 μm; Millipore, Merck ltd., Budapest, hungary) was coated with 5 μl of lipid solution (12 mg of porcine brain polar lipid extract dissolved in 420 μl hexane and 180 μl dodecane). the donor plate was inserted to the acceptor plate (Multiscreen acceptor Plate, MssaccePtOR; Millipore, Merck ltd., Budapest, hungary), which contained 300 μl of DPBs solution (ph 7.4). .. 150-150 μl of reference laM solution, as well as of the selected formulations redispersed with sNes (with a nominal concentration of 2 mg/ml of laM) were transferred to the donor plate lipid membrane. the sandwich system was incubated at 37 °c for 4 h (heidolph titramax 1000, heidolph instruments, schwabach, Germany). then, the PaMPa sandwich plates were separated and the laM concentrations in the donor and acceptor chambers were determined using RP-hPlc-DaD. the effective permeability and membrane retention of the drugs were calculated using the following eq. (3): P V A t C t Se A ss A 2 303 1 . . . log (3) where Pe is the effective permeability coefficient (cm/s), A is the filter area (0.24 cm2), VA is the volume of the acceptor phase (0.3 cm3), t is the incubation time (s), τSS is the time to reach steady state (s), CA(t) is the concentration of the compound in the acceptor phase at time point t (mol/cm3), and S is laM solubility in the donor phase. the solubility of laM in donor phase was determined after centrifugation (at 14,000 rpm, 15 minutes, eppendorf centrifuge 5804 R, thermo scientificTM, Waltham, Ma, Usa) in Microcon centrifugal Filter Devices (30,000 molecular weight (MWcO)) and 5× dilution of formulations in MeOh, using the same RP-hPlc-DaD system. the flux of the samples was calculated using the following eq. (4): Flux P S e = . (4) For each assay, six parallel measurements were made and data were presented as means ± sD. ..

Molecular Weight:

Article Title: Development, in vitro and ex vivo characterization of lamotrigine-loaded bovine serum albumin nanoparticles using QbD approach.
Article Snippet: In vitro permeability measurements to evaluate the permeability (Pe, cm/s) of the initial suspension and the selected formulations, the parallel artificial membrane permeability assay specific to the blood-brain barrier (BBB-PaMPa) was employed (Katona et al., 2020). a 10 mM laM solution was prepared in dimethylsulfoxide (DMsO), which was further diluted with sNes to obtain the 100 μM reference donor solution. the filter donor plate (MultiscreentM-iP, MaiPN4510, pore size 0.45 μm; Millipore, Merck ltd., Budapest, hungary) was coated with 5 μl of lipid solution (12 mg of porcine brain polar lipid extract dissolved in 420 μl hexane and 180 μl dodecane). the donor plate was inserted to the acceptor plate (Multiscreen acceptor Plate, MssaccePtOR; Millipore, Merck ltd., Budapest, hungary), which contained 300 μl of DPBs solution (ph 7.4). .. 150-150 μl of reference laM solution, as well as of the selected formulations redispersed with sNes (with a nominal concentration of 2 mg/ml of laM) were transferred to the donor plate lipid membrane. the sandwich system was incubated at 37 °c for 4 h (heidolph titramax 1000, heidolph instruments, schwabach, Germany). then, the PaMPa sandwich plates were separated and the laM concentrations in the donor and acceptor chambers were determined using RP-hPlc-DaD. the effective permeability and membrane retention of the drugs were calculated using the following eq. (3): P V A t C t Se A ss A 2 303 1 . . . log (3) where Pe is the effective permeability coefficient (cm/s), A is the filter area (0.24 cm2), VA is the volume of the acceptor phase (0.3 cm3), t is the incubation time (s), τSS is the time to reach steady state (s), CA(t) is the concentration of the compound in the acceptor phase at time point t (mol/cm3), and S is laM solubility in the donor phase. the solubility of laM in donor phase was determined after centrifugation (at 14,000 rpm, 15 minutes, eppendorf centrifuge 5804 R, thermo scientificTM, Waltham, Ma, Usa) in Microcon centrifugal Filter Devices (30,000 molecular weight (MWcO)) and 5× dilution of formulations in MeOh, using the same RP-hPlc-DaD system. the flux of the samples was calculated using the following eq. (4): Flux P S e = . (4) For each assay, six parallel measurements were made and data were presented as means ± sD. ..



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